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Master SPR for life sciences – from affinity and kinetic constants to conformation changes, hands-on training on a pre-selected set of molecules, SPR experiment planning, assay development, understanding of the underlying physico-chemical phenomena to resolve challenges, new applications of SPR from science and industry. You will become an independent expert in SPR.

WHAT WILL YOU LEARN: Master SPR for life sciences – from affinity and kinetic constants to conformation changes, hands-on training on a pre-selected set of molecules, SPR experiment planning, assay development, understanding of the underlying physico-chemical phenomena to resolve challenges, new applications of SPR from science and industry. You will become an independent expert in SPR.

LECTURERS:

Prof. Richard O’Kennedy (Dublin City University/Biotechnology), Prof. Vesa Hytönen (University of Tampere/Protein Dynamics), Dr. Tapani Viitala (University of Helsinki/Biopharmaceuticals), Dr. Arnoud Marquart (Sanquin Blood Supply Foundation, Founder of sprpages.nl), Prof. Jouko Peltonen (Åbo akademi/Physical chemistry), Prof. Peter Mattjus (Åbo akademi/Biochemistry),  As.prof. Karl Andersson (Uppsala university/Immunology, Ridgeview Instruments)

HANDS-ON TRAINING

- Antibody-antigen interactions
- And one of the following tracks based on your interests:

1. small drug molecules,
2. interaction of drugs with lipids,
3. interaction of drugs with living cells,
4. interaction on biomaterial interfaces, or
5. biomolecular quantification

From experiment planning, sensor surface selection, buffers, regeneration, through assay development, sample preparation and characterization to SPR measurements, data analysis and validation.

SUPPORTING LECTURES

• SPR history and theory introduced by one of the fathers of the technology
• SPR instrument configurations
• Sensor surfaces and immobilization strategies
• Measurement disturbances and artefacts (how does it show in measurements, theoretical reasoning for the effect, strategies to mitigate the effect)
• From sensogram to affinity and kinetic constants
• From measurement to conformation change
• Validation of results (ITC, AFM, TOF-SIMS,..)
• Application highlights from research and industry